Bio Engineering interview questions and answers - part 5

          

Bio Engineering interview questions and answers - part 5


Part 1   Part 2   Part 3   Part 4   Part 5   Part 6

Explain forbidden clones.

Clones refer to producing genetically identical individuals. Forbidden clones refer to clones of those cells which had immunological reactivity with self antigens. Embryonic life is eliminated from these types of clones. Such type of clones is called ‘forbidden clones’.

Explain gram staining method.

This method is used to identify bacterial species into two communities i.e. Gram positive and gram negative. This method is based on chemical and physical properties of their cell walls. It can be used to detect peptidoglycan, which is present in a thick layer in Gram positive bacteria. Purple/blue colour refers to the gram positive bacteria. Red colour stain refers to the gram negative bacteria. This method is very popularly used in the identification of bacterial organism.

Explain method of perfusion. State some of its drawbacks.

Perfusion is the process of delivery of blood to a capillary bed in the biological tissue. Tests of adequate perfusion are a part of the patient assessment process performed by medical or emergency personnel. The most common methods include evaluating skin color, temperature, condition and capillary refill. Perfusion can be of two types over perfusion and under perfusion. Types of perfusion is classified according to the average level of perfusion across all tissues in an individual body, Tissues like the heart are considered overperfused and receive more blood than would be expected to meet the metabolic needs of the tissue.

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